KMID : 0613820200300020113
|
|
Journal of Life Science 2020 Volume.30 No. 2 p.113 ~ p.121
|
|
Phellinus linteus Extract Regulates Macrophage Polarization in Human THP-1 Cells
|
|
Lee Sang-Yull
Park Sul-Gi Yu Sun-Nyoung Kim Ji-Won Hwang You-Lim Kim Dong-Seob Ahn Soon-Cheol
|
|
Abstract
|
|
|
Macrophages are initiators for regulating a host¡¯s defenses to eliminate pathogens and trigger tissue repair. Macrophages are classified into two types: classically (M1) activated macrophages and alternatively (M2) activated macrophages. M1-phenotype macrophages directly or indirectly kill infectious organisms and tumor cells via pro-inflammatory responses, whereas M2-phenotype macrophages remodel wounded tissue through anti-inflammatory responses. In this paper, we investigated how Phellinus linteus hot water extract passed from Diaion HP-20 resin (PLEP) regulates polarization of M1-like or M2-like macrophages in human THP-1 cells. PLEP did not have cytotoxicity at a high concentration of 300 ¥ìg/ml. We observed morphological alteration of the THP-1 cells, which are stimulated by PLEP, LPS/INF-¥ã (M1 stimulators) or IL-4/IL13 (M2 stimulators). PLEP exposure induced morphology contiguous with LPS/INF-¥ã. qPCR was also performed to determine whether PLEP influences M1 or M2 polarization-related genes. M1-phenotype macrophage?specific genes, such as TNF-¥á, IL-1¥â, IL-6, IL-8, CXCL10 and CCR7, were enhanced by PLEP in a dose-dependent manner similar to LPS/INF-¥ã. Conversely, M2-phenotype?specific genes, such as MRC-1, DC-SIGN, CCL17 and CCL22, were suppressed by PLEP. PLEP also significantly up-regulated secretory inflammation cytokines related to M1 polarization of macrophages, including TNF¥á, IL-1¥â and IL-6, which was similar to the gene expression. Further, MAPK and NF-¥êB signaling were increased by treatment with PLEP, resulting in enhancement of cytokine secretion. PLEP might therefore be used as a promising booster of pro-inflammatory responses through M1 polarization of human THP-1 cells.
|
|
KEYWORD
|
|
Human THP-1 cells, MAPK signaling, NF-¥êB signaling, Phellinus linteus extract, polarization of macrophage
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|